User:Elp3h.emily.powell/genetics groupwork

Genetics Group Work - Gene Project: Somatostatin receptor 2

Working as a group:

Group Member's Individual Research Topic:

• Nathan - Intro
• Dylan - Interactions
• Dominque - Structure
• Nick - (maybe? I dunno what exactly I should be writing...) not too sure either ?? Significance
  • http://www.med.harvard.edu/JPNM/TF94_95/Nov1/WriteUpNov1.html
  • https://www.ncbi.nlm.nih.gov/gene/6752
• Destiny - Function
• Emily - Discovery

To do list:

• really define somatostatin, receptor, and why it is "2"
  • break down the words (maybe this isn't a good idea??) I just think it would be pretty informative for the reader to have an idea of what words they are reading
• add links to difficult or confusing words
• how it is being studied
  • Somatostatin Receptor 2, or SST2, is being studied in by seeing its affect on inhibiting growth hormones and the duplication of cells.

Somatostatin Receptor 2

Nathan - Introduction:

The Somatostatin receptor 2 (SSTR2), which belongs to the G-protein coupled receptor family, is a gene that functions at many places in the body, including tumors, but has highest activity in the brain. In humans, SSTR2 activity is observed in the adrenal, brain, kidney, liver, prostate, stomach, spleen and many more body parts. SSTR2 can function as an inhibitor, receptor and even stimulate development to fulfill its purpose. The somatostatin receptor 2 is effective in that it keeps the systems of the body in check. SSTR2 is located on chromosome 17 on the long arm in position 25.1 in humans. This gene can be found in many species but is observed mainly in mammals.This gene functions in many animals including cows, pigs, mice, dogs, guinea pigs, wild yaks, and even some species of fish and amphibians. The SSTR2 gene is extremely useful in many areas and is especially observed in the medical field. SSTR2 is known to be useful for identification and even inhibition of tumors, cancers and other processes that get the body out of balance. Neuroendocrine tumors will over-express the somatostatin receptor 2 to either control the tumor or inhibit tumor growth completely. The expression of somatostatin receptor 2 in found in most tumors. The over-expression of somatostatin receptor 2 allows for greater ability to target certain signals in order to inhibit the tumor growth. SSTR2 is expressed in the central nervous system and pancreas and it also aids in neuroendocrine tumors by assisting to anticipate a reaction to radio-peptide therapy. SSTR2 is also very useful for many imaging studies used in medicine. Somatostatin receptor 2 also has the ability to stimulate apoptosis in many cells including cancer cells. SSTR2 has even been shown to have increased levels in people with certain cancers such as prostate and breast cancer. In this way, the somatostatin receptor 2 is somewhat of a defense system by inhibiting, receiving or stimulating when needed in order to keep the body in the correct condition. Cancer researchers are even looking at the somatostatin receptor 2 gene as a possible treatment in cancer patients because of its ability to inhibit tumor growths. Cancer researchers may look at exploiting the somatostatin receptor in cancer patients once it is determined how the somatostatin receptor interacts with cancer cells differently than with normal cells. The somatostatin receptor 2 is very effective and useful component in the body which may be utilized further in the medical field in the future.

Emily - Discovery:

There is a group of somatostatin receptors called the somatostatin receptor family. All of the members of the somatostatin receptor family are proteins that sit on the surface of the cell membrane and are responsible for the communication between cells . In 1972 , scientist were on the trek to discover more information on the hypothalamus and its "release factors." Studies showed patterns of inhibitory activity of the hypothalamus release factors which led scientists in the direction to discover somatostatin, known as the somatropin release-inhibiting factor, or SRIF. We now know that the SRIF is located at 3q28 (long arm of the third chromosome at the twenty-eighth position) in humans . Peering into location 3q28, the majority of proteins code for the pancreas, ovaries, and prostate along with other components of the endocrine system and nervous system , so it can be drawn that the receptor family has great influence among these systems. The family was first discovered in a segment of a rat's pituitary gland known as the tumor cell line http://www.guidetopharmacology.org/GRAC/FamilyIntroductionForward?familyId=61. A cell line is grown as a culture under controlled conditions, so the first discovery was found by culturing these cells in controlled conditions and in an environment outside of its norm. There, researchers found that the tumor cell line expresses a cell dividing inhibitor known as the transforming growth factor beta (TGF-beta) and also acts as an inhibitor to the milk producing hormone in female mammals, prolactin, and growth hormones. Researchers studied the activity of the receptors by conducting an assay with Ligand binding studies , which basically means they were conducting studies to see how prevalent the binding of the receptors occurred . Differences in how prevalently they receptors bonded revealed the existence of multiple receptors . Based on the ligand binding affinity and the receptors' signaling mechanisms, the receptor family was divided into 2 different groups, and within those groups, 5 subgroups. The group with a high affinity binding were classified under the SRIF1 group with sst2, sst3, and sst5 in the subgroup, while the receptors with low affinity binding were classified under the SRIF2 group with sst1 and sst4 in the subgroup. Manipulations with the somatostatin receptors are used for many therapies in both the endocrine and nervous system, and now that we know the groups and subgroups of the receptor family, therapy treatment is much more efficient and effective. For example, as you continue reading the article, you will notice the importance and advancements of oncology and tumor treatments, as well as other ways the somatostatin receptors are working and advancing the world of medicine. .

According to https://www.ncbi.nlm.nih.gov/gene/6752 , the somatostatin receptor 2 is found on the chromosome 17 (chart below is directly from https://www.ncbi.nlm.nih.gov/gene/6752, copywrite infringement not intended). Information was gathered and determined from a sample of individuals, and conclusions were drawn upon location and other information regarding the SSRT2 protein .

Gene:	SSTR2
Title:	somatostatin receptor 2
Location:	73,165,021..73,171,955
Length:	6,935 nt
[Positional Info]
NC_000017.11 position:	73,168,608
Gene position:	3,588

Nick - Significance :

The somatostatin hormone itself can negatively affect the uptake of hormones in the body and may play a role in some hormonal conditions. Somatostatin 2 receptors have been found in concentration on the surface of tumor cells, particularly those associated with the neuroendocrine system where the overexpression of somatostatin can lead to many complications. Due to this, these receptors are considered a prospective aid for the detection of tumors, especially in patients who present with conditions like hypothyroidism and Cushing’s syndrome. A synthetic version of the somatostatin hormone, octreotide, has been successfully used in combination with radio-peptide tracers to locate adrenal gland tumors through scintigraphic imaging. A similar method may be utilized to carry and more accurately administer radioactive treatments to tumors. Octreotide and other analogs are preferred for this use due to their possessing of an extended half life compared to the naturally-occurring hormone allowing for more flexibility when used for such treatments.

The association of somatostatin 2 receptors on tumors has also lead to the suggestion of possible alternatives to current tumor treatment methods. The binding of synthetic somatostatin hormones such as octreotide to receptors has been seen to reduce the production of hormones and is now being considered for use in the treatment of some pituitary tumors. One group suggests that the treatment method would be particularly effective against thyrotropin-secreting pituitary adenomas (TSHomas), though further inquiries and clinical trials are needed.

SSTR2 is also being investigated for its potential use as a reporter gene in for the visualization of regional gene expression. One study tested this by comparing the PET/CT and light imaging results of laboratory rats’ musculature obtained through the use of a human somatostatin receptor 2 vector and a control luciferase vector. The study suggests that somatostatin receptor genes could be an effective substitute for the current viral-based vectors since the sstr genes elicits less of an immune response and has overall been well-tolerated by the trial patients’ bodies. This form of treatment may be especially useful for the study of gene expression in larger mammals whose larger body mass may obstruct clear visualization of deep tissue areas. The use of sstr2 and sstr5 as biomarkers to track the progress of and treat neuroendocrine tumors displaying circulating tumor cells is also being investigated due to these cells’ somatostatin receptor gene expressivity.