User:Thanapol Nongbua(SLU)
Thanapol Nongbua | |
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| Occupation | PhD student at SLU |
| Website | SLU Wikipedia Project |
About me
[edit]Hi,
My name is Thanapol Nongbua and I am a PhD student at SLU. My area of research is Bovine seminal plasma in fertility and I conduct most of my research at the Department of Food Science. You may find my personal page on SLU here. Please find my userpage on other Wikipedia language versions in the menu to the left.
My contributions on Wikipedia
[edit]You may easily find my contributions on Wikipedia by clicking here.
I have initiated these articles
[edit]Example 1
Relationship between 56-day non-return rate and the quality of frozen thawed bull semen
Thanapol Nongbua1,3, Lavanya Goodla1, Anders Johannisson2, Jane Morrell1
1Division of Reproduction, Swedish University of Agricultural Sciences(SLU) Box 7054, SE-750 07 Uppsala Sweden
2Department of Anatomy,Physiology and Biochemistry (SLU) Box 7011, SE-750 07 Uppsala Sweden
3Department of Clinical Sciences, Faculty of Veterinary Sciences, Mahasarakham University, Mahasarakham, 44000 Thailand
The most common of evaluating semen in the field is by evaluating sperm motility although a combination of parameters of sperm quality may be a better indicator of fertility (1). The objective of this study was to investigate the relationship between 56-day non-return rate (NRR) and the quality of frozen thawed bull semen. Method: From 24 bulls, semen samples frozen in Andromed extender (Minitűb, Tiefenbach, Germany) were available for analysis. Field fertility data were available from these bull based on the 56 day NRR from 40,185 artificial inseminations, indexed to account for factors such as inseminator, farm, area, age of female (2). The straws were thawed in water (37ºC) for 12 sec. Aliquots from one straw were used for the following evaluations: Flow cytometric determination of membrane integrity, chromatin integrity, mitochondrial membrane potential, reactive oxygen species (ROS) and protein tyrosine phospholyration. Correlations between 56-day NRR and the sperm quality were analysed using the statistical software in the Excel package (Version 2010, Microsoft Corporation, Redmond, WA, USA). The 56-day NRR was positively correlated with membrane integrity for living sperm(r=0.4760 P<0.05) and negatively correlated with dead sperm (r=-0.5307 P<0.05),negatively correlated with %DNA fragmentation index (r=-0.5194 P<0.05) and Mean_DNA fragmentation index (r=-0.5210 P<0.05). There were no significant correlations for the remaining parameters. Conclusion: The results suggest sperm viability and chromatin integrity may be useful for the prediction of field fertility.
References:
1. Colenbrander et al., (2003) Reprod Domest Anim 38, 305–311.
2. Stålhammar et al. (1994) Reprod Nutr Dev. 1994;34:37-45