User:Daniel Mietchen/Sandbox/Cameleon

Modified copy from
Costa, A.; Candeo, A.; Fieramonti, L.; Valentini, G.; Bassi, A. (2013). Bennett, Malcolm (ed.). "Calcium Dynamics in Root Cells of Arabidopsis thaliana Visualized with Selective Plane Illumination Microscopy". PLoS ONE. 8 (10): e75646. doi:10.1371/journal.pone.0075646. PMID 24146766.{{cite journal}}: CS1 maint: unflagged free DOI (link)

Cameleons are Förster Resonance Energy Transfer (FRET) based indicators in which two fluorescent proteins, CFP and YFP (or circularly permuted variants of YFP), are linked together by the calcium-binding protein calmodulin and a calmodulin binding peptide. Binding of Ca2+ to these calcium-responsive elements alters the distance between the two fluorophores, hence the efficiency of FRET, allowing a quantitative measurement of Ca2+ dynamics [21].