Talk:Defective Interfering RNA

Defective Interfering RNA Topic Peer Review 1

Content

To start off, great job on updating the information that is currently on Wikipedia about T-Box Leader RNA. In comparison to what is already there, your group has successfully offered a much better summary and description of the RNA element. The specific subsections were wisely chosen and are important in gaining a comprehensive understanding of the topic. The information that is provided in the Introductory/ Background section is good, however, there is room for improvement. Overall, the audience defiantly gets a good idea of what the topic is about. However, some terminology and details can be further explained to make it more accessible to non-scientists. The following are just suggestions and could be incorporated if you choose. In the first sentence, you state “..are mutated sub-viral RNAs" what are mutated-subviral RNAs? Why do they play a role in the evolution of viral diseases? Also, perhaps give examples of terms used like viral replication and "Coronavirus EL..”. These terms may be familiar to us because we have read about them frequently in textbooks, but may not be to non-sceicnets, so more information could help. But overall, the introduction is a great start!

Overall, the contents of each section justify the lengths of the individual sections. However, some more information in some of the sections may be needed to fully understand the topic. For example, in the Defection section, perhaps where you state that they lack the genes for independent replication, add some sources of research articles that are studying it and what they have so far uncovered about this. Also, the sentence “They usually retina the ends of non-defective RNA but never retain their own replicase,” perhaps include the source and provide more detail so the audience can better understand. The “Types of Defective Genomes” section is done well, but there are no sources for this information. Also, examples of each, rather then just listing them would be helpful. There is also no image to your page, perhaps an image in this section illustrated the type sod defective genomes would be helpful. In the “Interference” section, more details may be necessary to be able to understand more comprehensively. For example, when you say that it is becoming more and more important for future research therapies, perhaps include some of these therapies to give the audience more information. In addition, maybe an image would be helpful in this section. The “Recent Published Work” section could also use information. For example, you state that there has been a lot of recent work done by virologists to learn more about interference in infection of host cells, but adding specifics about this is important. Also, just defining some key words or adding examples would help for terms like “ sucrose-gradient preparation” and “antiviral agents”.

To continue, the article has a lot of terms that are further explained in various other Wikipedia pages. Currently, the terms are linked to their respective pages on Wikipedia. This is helpful because it give the audience an opportunity to learn more about your topic and spark interest in other topics.

Overall, your group effectively provided sufficient information about Defective Interfering RNA. The information provided was very interesting and is able to expand the current page to a great extent. There wasn’t very much repeat in comparison to the current page, besides the image and a little from the introduction, but, in my opinion, this information was necessary.

Figures

There is currently no figure chosen to further explain the topic. Perhaps adding an image for the introduction would help. If your group chooses to expand on the structure, it could be helpful to include an image here. In addition, as I have previously mentioned, an image in the “Types of Defective Genomes” section could also be useful in helping the audience better visualize the different types. In addition, your group could add an image to the interference section to explain competitive inhibition.

References

Your group has only five sources for the information provided, not meeting the minimum of 10 required. They were correctly incorporated into the text and formatted in the correct fashion at the bottom of the page. However, your group only includes sources from journals. It is important to have a variety of different types of sources. Perhaps include some information from printed textbooks or other non-journal sources! Finally, to meet the source requirement, it will be essential to add more details to the specific sections with examples. Perhaps these examples can be from non-jounral sources.

Overall Presentation

The group did a great job overall for the first draft in concicly explaining what Defective Interfering RNA is and the relevant information about it. For example, the introduction section effectively explains what the topic is, but it may be helpful to add more information/details about some terms to make it more understandable to non-scienctists. To continue, the individual sections chosen by the group were effective in further expanding on the topic and provided other necessary details. However, the group should pay more attention to the words used and to define, give further explanation, and provide external examples to make it more accessible to non-scientists. In addition, there was no image provided. Adding an image is essential, and specific areas where images could be added are explained above Next, the group did a great job of adding links to external Wikipedia pages to help the audience gain more information about some of the terms used in the article. This helps readers gain more information about the topic as well as allow them to learn about other topics. The overall writing style was effective and professional, great job in avoiding informal words. Overall, great job on this article, it is a very interesting topic and I look forward to reading your final draft!

Arshpatel

Defective Interfering RNA Topic Peer Review 2

Content

Before reading this Wikipedia article and your updated version, I knew nothing about the topic, so it was interesting to learn a little bit more about DI-RNA! I like how you changed and expanded upon the introduction of the previously existing Wiki article's introduction. While reading the introduction, however, I did feel as though the sentences could have been better organized. For example, it might make more sense to define what DI-RNA is generally, where it is found, how it is formed and replicates, and then go into specifics of what stipulates DI-RNA. I did feel as though the introduction was pretty accessible to a non-expert but there were a couple terms that could use some clarification. For example, defining what "subviral RNA" might be helpful. The sentence "DI-RNAs contain viral structural proteins but are missing a part of the virus's genome." was confusing to me so perhaps expanding that into two sentences could help explain what you mean.

Overall, I thought the organization of the various sections was pretty appropriate although I would like to see more information added to give length and depth. It appears as though "Types of Defective Genomes" could have been a subcategory under the main heading "Defection." I think these two categories can also be expanded in length by not just detailing how one categories something as DI-RNA but how this happens. Again, with the category labeled "Interference," it might be interested to expand the length of this section by giving specific examples of when this happens and what the molecular basis for this happening is.

Most important terms and concepts are linked to their respective Wiki links, although in the "Recent Published Works" section, it will be helpful - when more information is added - to include links explaining the different methods that were employed in the research. Since the original wiki article is so sparse, the content you have thus provided is original and not duplicated anywhere else.

Figures

There are not yet any figures included in your draft, but I have a couple suggestions of some figures that might be helpful to find. It would be helpful to have a figure that compared DI-RNA and normal viral RNA to specifically point out what makes DI-RNA different, since a lot of the information you have provided point out these differences. If no figures are available, you might be able to also show the differences using Chemdraw. Another figure that might be helpful is one that could accent the "Types of Defective Genomes" section because the reader could visualize the

References

The reference section is not yet complete, but will be benefited with the addition of more information. The way in which you included the references and cited them in the paragraphs was correct. A way to non-journal sources might be through the inclusion of specific examples of DI-RNAs.

Overall Presentation

Overall, I think your Wikipedia draft for DI-RNA is off to a great start. I think all of the sections that you chose to work on are important topics. I especially liked your inclusion of the last section titled "Recent published works." Because this topic is still being researched, I think having this section towards the end is important because it gives the reader new insights into how the field might change moving forward. Moving forward, I think it would be helpful to reevaluate each section and determine how you can expand and fill out each section. For example, adding more information and background history to the introduction or giving specific examples and more information on DI-RNA's molecular basis during the rest of the body paragraphs. The addition of some figures would do well to help the reader understand how DI-RNA is different from other molecules we may already be familiar with.

rneelam

Suggestions from ChemLibrarian

Good work! Please address issues mentioned by reviewers. Some detailed explanations in each section and a figure would be great.

Your internal link error of the Defective Interfering Particles can be fixed this way [[Defective interfering particle]. THe capitalization in wikipedia article titles matters. Please add more internal links throughout the text.

Please fix the (NEED MORE INFORMATION) part of the last section Recent Published Works. I'd also suggest changing the title of this section according to the content.

Hope it helps! Please let me know if you have any questions. ChemLibrarian (talk) 16:01, 27 October 2014 (UTC)

GSI Comments

Thank you for your addition to this page. Please take into consideration the comments from the reviewers. With a few edits I think you will have a great page!

Elizabeth — Preceding unsigned comment added by ChemStudent24601 (talk • contribs) 17:13, 27 October 2014 (UTC)

response to reviews

Thank you all for your great reviews of the page. We are working on finding an image to use and will upload one shortly. Thank you for the suggestions about how to make the material more accessible for non-scientists and also to give examples of the different defection types. We are working to address these. Dutcherh — Preceding unsigned comment added by Dutcherh (talk • contribs) 16:45, 28 October 2014 (UTC)