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tRNA (guanine-N1-)-methyltransferase

From Wikipedia, the free encyclopedia
tRNA (guanine-N1-)-methyltransferase
Identifiers
EC no.2.1.1.31
CAS no.37256-99-8
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO
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PMCarticles
PubMedarticles
NCBIproteins

In enzymology, a tRNA (guanine-N1-)-methyltransferase (EC 2.1.1.31) is an enzyme that catalyzes the chemical reaction

S-adenosyl-L-methionine + tRNA S-adenosyl-L-homocysteine + tRNA containing N1-methylguanine

Thus, the two substrates of this enzyme are S-adenosyl methionine and tRNA, whereas its two products are S-adenosylhomocysteine and tRNA containing N1-methylguanine.

This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is S-adenosyl-L-methionine:tRNA (guanine-N1-)-methyltransferase. Other names in common use include transfer ribonucleate guanine 1-methyltransferase, tRNA guanine 1-methyltransferase, and S-adenosyl-L-methionine:tRNA (guanine-1-N-)-methyltransferase.

Structural studies

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As of late 2007, 6 structures have been solved for this class of enzymes, with PDB accession codes 1OY5, 1P9P, 1UAJ, 1UAK, 1UAL, and 1UAM.

References

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  • Hurwitz J, Gold M, Anders M (1964). "The Enzymatic Methylation of Ribonucleic Acid and Deoxyribonucleic Acid. 3. Purification of Soluble Ribonucleic Acid-Methylating Enzymes". J. Biol. Chem. 239: 3462–3473. doi:10.1016/S0021-9258(18)97746-X. PMID 14245404.
  • Bjork GR, Svensson I (1969). "Studies on microbial RNA. Fractionation of tRNA methylases from Saccharomyces cerevisiae". Eur. J. Biochem. 9 (2): 207–215. doi:10.1111/j.1432-1033.1969.tb00596.x. PMID 4896260.
  • Smolar N, Hellman U, Svensson I (1975). "Two transfer RNA (1-methylguanine) methylases from yeast". Nucleic Acids Res. 2 (6): 993–1004. doi:10.1093/nar/2.6.993. PMC 343485. PMID 1096087.
  • Glick JM, Averyhart VM, Leboy PS (1978). "Purification and characterization of two tRNA-(guanine)-methyltransferases from rat liver". Biochim. Biophys. Acta. 518 (1): 158–171. doi:10.1016/0005-2787(78)90125-9. PMID 629973.