RuvABC
 | This article includes a list of general references, but it lacks sufficient corresponding inline citations. (October 2017) |
RuvABC is a complex of three proteins that mediate branch migration and resolve the Holliday junction created during homologous recombination in bacteria. As such, RuvABC is critical to bacterial DNA repair.
RuvA and RuvB bind to the four strand DNA structure[1] formed in the Holliday junction intermediate, and migrate the strands through each other, using a putative spooling mechanism. The RuvAB complex can carry out DNA helicase activity, which helps unwind the duplex DNA. The binding of the RuvC protein to the RuvAB complex is thought to cleave the DNA strands, thereby resolving the Holliday junction.
Protein complex
[edit]The RuvABC is a complex of three proteins that resolve the Holliday junction formed during bacterial homologous recombination. In Escherichia coli bacteria, DNA replication forks stall at least once per cell cycle, so that DNA replication must be restarted if the cell is to survive.[2] Replication restart is a multi-step process in E. coli that requires the sequential action of several proteins. When the progress of the replication fork is impeded the proteins single-stranded binding protein SSB and RecG helicase along with the RuvABC complex are required for rescue.[2] The resolution of Holliday junctions that accumulate following replication on damaged DNA templates in E. coli requires the RuvABC complex.[3]
RuvA
[edit]RuvA (Holliday junction branch migration complex subunit RuvA)[4] is a DNA-binding protein that binds Holliday junctions with high affinity. The structure of the complex has been variously elucidated through X-ray crystallography and EM data, and suggest that the complex consists of either one or two RuvA tetramers, with charge lined grooves through which the incoming DNA is channelled. The structure also showed the presence of so-called 'acidic pins' in the centre of the tetramer, which serve to separate the DNA duplexes. Its crystal structure has been solved at 1.9A.
RuvB
[edit]RuvB (Holliday junction branch migration complex subunit RuvB)[5] is an ATPase that is only active in the presence of DNA and compared to RuvA, RuvB has a low affinity for DNA. The RuvB proteins are thought to form hexameric rings on the exit points of the newly formed DNA duplexes, and it is proposed that they 'spool' the emerging DNA through the RuvA tetramer.
RuvC
[edit]RuvC (Crossover junction endodeoxyribonuclease RuvC)[6] is the resolvase, which cleaves the Holliday junction. RuvC proteins have been shown to form dimers in solution and its structure has been solved at 2.5A. It is thought to bind either on the open, DNA exposed face of a single RuvA tetramer, or to replace one of the two tetramers. Binding is proposed to be mediated by an unstructured loop on RuvC, which becomes structured on binding RuvA. RuvC can be bound to the complex in either orientation, therefore resolving Holliday junctions in either a horizontal or vertical manner.
See also
[edit]References
[edit]- ^ "RuvA - Holliday junction ATP-dependent DNA helicase RuvA - Thermus thermophilus (Strain ATCC 27634 / DSM 579 / HB8) - ruvA gene & protein".
- ^ a b Bianco PR, Lu Y (May 2021). "Single-molecule insight into stalled replication fork rescue in Escherichia coli". Nucleic Acids Res. 49 (8): 4220–4238. doi:10.1093/nar/gkab142. PMC 8096234. PMID 33744948.
- ^ Donaldson JR, Courcelle CT, Courcelle J (September 2006). "RuvABC is required to resolve holliday junctions that accumulate following replication on damaged templates in Escherichia coli". J Biol Chem. 281 (39): 28811–21. doi:10.1074/jbc.M603933200. PMID 16895921.
- ^ "RuvA". www.uniprot.org.
- ^ "RuvB". www.uniprot.org. Retrieved 6 April 2024.
- ^ "RuvC". www.uniprot.org. Retrieved 6 April 2024.
Further reading
[edit]- West SC (2003). "Molecular views of recombination proteins and their control". Nat. Rev. Mol. Cell Biol. 4 (6): 435–45. doi:10.1038/nrm1127. PMID 12778123. S2CID 28474965.
- Kowalczykowski SC (2000). "Initiation of genetic recombination and recombination-dependent replication". Trends Biochem. Sci. 25 (4): 156–65. doi:10.1016/S0968-0004(00)01569-3. PMID 10754547.
- Eggleston AK, Mitchell AH, and West SC (1997). “In Vitro Reconstitution of the Late Steps of Genetic Recombination in E. coli”. Cell. 89: 607–617.
External links
[edit]- Holliday+Junction+Resolvases at the U.S. National Library of Medicine Medical Subject Headings (MeSH)