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Summary

Description
English: Within the organisms, genes are transcribed and spliced (in eukaryotes) to produce mature mRNA transcripts (red). The mRNA is extracted from the organism, fragmented and copied into stable ds-cDNA (blue). The ds-cDNA is sequenced using high-throughput, short-read sequencing methods. These sequences can then be aligned to a reference genome sequence to reconstruct which genome regions were being transcribed. This data can be used to annotate where expressed genes are, their relative expression levels, and any alternative splice variants.[1]
Date
Source Own work
Author Thomas Shafee
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Also in series:

  • RNA Microarray
    RNA Microarray
  • RNA-Seq
    RNA-Seq
  • SAGE
    SAGE

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  1. ↑ Lowe, Rohan (2017-05-18). "Transcriptomics technologies". PLOS Computational Biology 13 (5): e1005457. DOI:10.1371/journal.pcbi.1005457. PMID 28545146. PMC: PMC5436640. ISSN 1553-7358.

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RNA sequencing

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5 October 2016

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Date/TimeThumbnailDimensionsUserComment
current12:20, 13 February 2017Thumbnail for version as of 12:20, 13 February 20171,020 × 803 (246 KB)Evolution and evolvabilitytext as paths to avoid artefacts
12:19, 13 February 2017Thumbnail for version as of 12:19, 13 February 20171,020 × 803 (90 KB)Evolution and evolvabilitytext as paths to avoid artefacts
10:48, 13 February 2017Thumbnail for version as of 10:48, 13 February 20171,020 × 803 (88 KB)Evolution and evolvabilityupdate sequence text to 'sans-serif' open font for more robust rendering
08:41, 10 February 2017Thumbnail for version as of 08:41, 10 February 20171,020 × 803 (87 KB)Evolution and evolvabilityreverse order of fragmentation and reverse-transcription
05:08, 5 October 2016Thumbnail for version as of 05:08, 5 October 20161,020 × 803 (85 KB)Evolution and evolvabilityUser created page with UploadWizard

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