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English: Simplified scheme of shotgun sequencing: firstly, the DNA fragment is cut into small, overlapping pieces. Then each fragment is sequenced, and the complete sequence is assembled based on the similarity of the overlapping ends. This approach can be applied only to small genomes or fragments of bigger genomes due to the presence of multiple repeated sequences.
Polski: Uproszczony schemat sekwencjonowania metodą "shotgun": najpierw fragment DNA jest poddawany fragmentaryzacji na małe, zachodzące na siebie kawałki. Następnie każdy fragment jest sekwencjonowany, a cała sekwencja jest składana na podstawie podobieństwa zachodzących na siebie końców. To podejście można stosować tylko do małych genomów lub fragmentów większych genomów ze względu na obecność sekwencji wielokrotnie powtórzonych.
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Author Bumblingbiologist

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Simplified scheme of shotgun sequencing technique

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30 December 2024

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