CXorf58
| CXorf58 | |||||||||||||||||||||||||||||||||||||||||||||||||||
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| Identifiers | |||||||||||||||||||||||||||||||||||||||||||||||||||
| Aliases | CXorf58, chromosome X open reading frame 58 | ||||||||||||||||||||||||||||||||||||||||||||||||||
| External IDs | HomoloGene: 82308; GeneCards: CXorf58; OMA:CXorf58 - orthologs | ||||||||||||||||||||||||||||||||||||||||||||||||||
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| Wikidata | |||||||||||||||||||||||||||||||||||||||||||||||||||
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Human chromosome X open reading frame 58 (CXorf58) is a protein coding gene encoding the CXorf58 protein.[3]
Gene
[edit]CXorf58 contains 9 exons. CXorf58 is 31,578 nucleotides long. The longest exon in CXorf58 is exon 1, containing 324 nucleotides and the shortest exon is exon 4, containing 111 nucleotides. CXorf58 is highly expressed in sperm cells, particularly at the lower point of the head and midpiece of spermatids.[4]
Isoforms
[edit]There are two isoforms for CXorf58 protein. Isoform 1 for CXorf58 is 332 amino acids long and isoform 2 is 330 amino acids long. Isoform 2 uses an alternate in-frame splice site in the 3' coding region, resulting in a shorter protein.[3]
Protein
[edit]
The molecular weight of CXorf58 isoform 1 protein is 38.9kDa.[6] It is rich in basic amino acids and abundant in the amino acids methionine and arginine.[7] CXorf58 contains 3 asparagine-linked glycosylation sites and 5 phosphorylation sites.[8]
Gene level regulation
[edit]CXorf58 contains a tissue-specific expression pattern. It is found in high abundance in the testis.[3]
Protein level regulation
[edit]CXorf58 is localized in the cytoplasm of cells.[9] CXorf58 contains 10 net phosphorylation sites. There is one glycosylation site for CXorf58 at amino acid 320.[10]
Homology
[edit]Orthologs of CXorf58 appear in an array of taxonomic groups.
| Species | Common Name | NCBI Accession Number[11] | Sequence Length (amino acids) | Date of Divergence (MYA) | Sequence Identity (%) | Sequence Similarity (%) |
|---|---|---|---|---|---|---|
| Homo sapiens | Human | XP_054182731.1 | 332 | - | - | - |
| Gorilla gorilla gorilla | Western lowland gorilla | XP_004063966.2 | 332 | 8.6 | 99% | 99% |
| Sciurus carolinensis | Eastern gray squirrel | XP_047392635.1 | 370 | 87 | 62% | 69% |
| Mus Musculus | House mouse | NP_001349810.1 | 368 | 87 | 56% | 65% |
| Equus quagga | Plains zebra | XP_046529678.1 | 374 | 94 | 64% | 71% |
| Panthera uncia | Snow leopard | XP_049499554.1 | 380 | 94 | 64% | 66% |
| Cervus canadensis | Elk | XP_043314584.1 | 372 | 94 | 63% | 69% |
| Bos taurus | Cattle | XP_024843724.1 | 370 | 94 | 63% | 68% |
| Struthio camelus australis | South African ostrich | XP_009663695.1 | 342 | 319 | 49% | 54% |
| Dromaius novaehollandiae | Emu | XP_025959174.1 | 334 | 319 | 48% | 53% |
| Nothoprocta perdicaria | Chilean tinamou | XP_025894453.1 | 332 | 319 | 47% | 51% |
| Chrysemys picta bellii | Painted turtle | XP_065435515.1 | 408 | 319 | 48% | 53% |
| Chelonia mydas | Green sea turtle | XP_037757767.1 | 353 | 319 | 48% | 54% |
| Danio rerio | Zebrafish | XP_021332053.1 | 253 | 429 | 44% | 65% |
| Asterias rubens | Common starfish | XP_033637843.1 | 404 | 619 | 44% | 53% |
| Saccoglossus kowalevskii | Acorn worm | XP_002736551.1 | 390 | 619 | 44% | 53% |
| Ruditapes philippinarum | Manila clam | XP_060606373.1 | 446 | 686 | 45% | 42% |
| Lineus longissimus | Bootlace worm | XP_064639061.1 | 393 | 686 | 45% | 52% |
| Liolophura japonica | Chiton liolophura japonica | XP_064615141.1 | 435 | 686 | 45% | 45% |
| Patella vulgata | Common limpet | XP_050389200.1 | 434 | 686 | 41% | 45% |
Interacting proteins
[edit]Five proteins have interactions with CXorf58: GDE1, H2BC9, HOXA1, TGFBR2, and DCAF5.[12]
| Protein | Protein Full Name[13] | Function[13] |
|---|---|---|
| GDE1 | Glycerophosphodiester Phosphodiesterase 1 | Enables glycerophosphodiester phosphodiesterase activity, glycerophosphoinositol glycerophosphodiesterase activity, and lysophospholipase activity. |
| H2BC9 | H2B Clustered Histone 9 | Encodes a replication-dependent histone that is a member of the histone H2B family. |
| HOXA1 | Homeobox A1 | Encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. |
| TGFBR2 | Transforming Growth Factor Beta Receptor 2 | Forms a heterodimeric complex with TGF-beta receptor type-1, and binds TGF-beta. |
| DCAF5 | DDB1 and CUL4 Associated Factor 5 | Involved in protein ubiquitination. |
Clinical significance
[edit]Mutations that occur on CXorf58 include deletions of base pairs, missense mutations, nonsense mutations, and frameshift mutations. Many of which are coding sequence variants.[14]
References
[edit]- ^ a b c GRCh38: Ensembl release 89: ENSG00000165182 – Ensembl, May 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ a b c "CXorf58 chromosome X open reading frame 58 [Homo sapiens (human)] - Gene - NCBI". www.ncbi.nlm.nih.gov. Retrieved 2024-09-21.
- ^ Vandenbrouk, Yves (July 22, 2016). "Looking for Missing Proteins in the Proteome of Human Spermatozoa: An Update". Journal of Proteome Research. 15 (11): 3998–4019. doi:10.1021/acs.jproteome.6b00400 – via ACS Publications.
- ^ "I-TASSER server for protein structure and function prediction". zhanggroup.org. Retrieved 2024-09-26.
- ^ "CXorf58 Gene - Chromosome X Open Reading Frame 58". GeneCards.
- ^ "EMBL-EBI SAPS". EMBL's European Bioinformatics Institute.
- ^ "myhits Motif Scan". myhits.
- ^ "DTU Health Tech DeepLoc - 2.0". DTU Health Tech.
- ^ "DTU Health Health Tech Department of Health Technology Services". DTU Health Tech.
- ^ "National Center for Biotechnology Information". NCBI.
- ^ "IntAct". EMBL's European Bioinformatics Institute.
- ^ a b "GeneCards". GeneCards.
- ^ "NCBI Variation Viewer". NCBI.